Les protéines du choc thermique
Avec l’équipe du Pr Robert Tanguay à l’Université Laval à Québec, nous avons étudié les protéines du choc thermique qui sont des protéines exprimées lors des stress cellulaires extrêmes. Il y en a plusieurs qui ont par la suite été identifiées comme des protéines chaperonnes.
Members of the Drosophila HSP 70 family share ATP-binding properties. Beaulieu JF, Tanguay RM. Eur J Biochem. 1988 Mar 1;172(2):341-7. https://pubmed.ncbi.nlm.nih.gov/3350001/
Interaction of Drosophila 27,000 Mr heat-shock protein with the nucleus of heat-shocked and ecdysone-stimulated culture cells. Beaulieu JF, Arrigo AP, Tanguay RM. J Cell Sci. 1989 Jan;92 ( Pt 1):29-36. https://pubmed.ncbi.nlm.nih.gov/2777913/
Intramitochondrial localization of the main 70-kDa heat-shock cognate protein in Drosophila cells. Carbajal EM, Beaulieu JF, Nicole LM, Tanguay RM. Exp Cell Res. 1993 Aug;207(2):300-9. https://pubmed.ncbi.nlm.nih.gov/8344382/
Le métabolisme des lipides
Notre laboratoire collabore depuis plusieurs années avec le Pr Emile Levy et les membres de son équipe pour améliorer nos connaissances sur le métabolisme des lipides, dans l’intestin qui constitue la porte d’entrée, et dans les maladies qui y sont reliées.
Apolipoproteins in human fetal colon: immunolocalization, biogenesis, and hormonal regulation. Basque JR, Lévy E, Beaulieu JF, Ménard D. J Cell Biochem. 1998 Sep 1;70(3):354-65. https://pubmed.ncbi.nlm.nih.gov/9706873/
Human crypt intestinal epithelial cells are capable of lipid production, apolipoprotein synthesis, and lipoprotein assembly. Levy E, Beaulieu JF, Delvin E, Seidman E, Yotov W, Basque JR, Ménard D. J Lipid Res. 2000 Jan;41(1):12-22. https://pubmed.ncbi.nlm.nih.gov/10627497/
Intestinal-fatty acid binding protein and lipid transport in human intestinal epithelial cells. Montoudis A, Delvin E, Menard D, Beaulieu JF, Jean D, Tremblay E, Bendayan M, Levy E. Biochem Biophys Res Commun. 2006 Jan 6;339(1):248-54. https://pubmed.ncbi.nlm.nih.gov/16297872/
Localization and role of NPC1L1 in cholesterol absorption in human intestine. Sané AT, Sinnett D, Delvin E, Bendayan M, Marcil V, Ménard D, Beaulieu JF, Levy E. J Lipid Res. 2006 Oct;47(10):2112-20. https://pubmed.ncbi.nlm.nih.gov/16829661/
Subcellular proteomics of cell differentiation: quantitative analysis of the plasma membrane proteome of Caco-2 cells. Pshezhetsky AV, Fedjaev M, Ashmarina L, Mazur A, Budman L, Sinnett D, Labuda D, Beaulieu JF, Ménard D, Nifant’ev I, Levy E. Proteomics. 2007 Jun;7(13):2201-15. https://pubmed.ncbi.nlm.nih.gov/17549793/
Effect of retinoic acid on cell proliferation and differentiation as well as on lipid synthesis, lipoprotein secretion, and apolipoprotein biogenesis. Grenier E, Maupas FS, Beaulieu JF, Seidman E, Delvin E, Sane A, Tremblay E, Garofalo C, Levy E. Am J Physiol Gastrointest Liver Physiol. 2007 Dec;293(6):G1178-89. https://pubmed.ncbi.nlm.nih.gov/17916647/
Intestinal fatty acid binding protein regulates mitochondrion beta-oxidation and cholesterol uptake. Montoudis A, Seidman E, Boudreau F, Beaulieu JF, Menard D, Elchebly M, Mailhot G, Sane AT, Lambert M, Delvin E, Levy E. J Lipid Res. 2008 May;49(5):961-72. https://pubmed.ncbi.nlm.nih.gov/18235139/
Functional development of human fetal gastrointestinal tract. Lévy E, Delvin E, Ménard D, Beaulieu JF. Methods Mol Biol. 2009;550:205-24. https://pubmed.ncbi.nlm.nih.gov/19495706/
Localization, function and regulation of the two intestinal fatty acid-binding protein types. Levy E, Ménard D, Delvin E, Montoudis A, Beaulieu JF, Mailhot G, Dubé N, Sinnett D, Seidman E, Bendayan M. Histochem Cell Biol. 2009 Sep;132(3):351-67. https://pubmed.ncbi.nlm.nih.gov/19499240/
Modification in oxidative stress, inflammation, and lipoprotein assembly in response to hepatocyte nuclear factor 4alpha knockdown in intestinal epithelial cells. Marcil V, Seidman E, Sinnett D, Boudreau F, Gendron FP, Beaulieu JF, Ménard D, Precourt LP, Amre D, Levy E. J Biol Chem. 2010 Dec 24;285(52):40448-60. https://pubmed.ncbi.nlm.nih.gov/20871093/
Antioxidative properties of paraoxonase 2 in intestinal epithelial cells. Précourt LP, Marcil V, Ntimbane T, Taha R, Lavoie JC, Delvin E, Seidman EG, Beaulieu JF, Levy E. Am J Physiol Gastrointest Liver Physiol. 2012 Sep 1;303(5):G623-34. https://pubmed.ncbi.nlm.nih.gov/22744335/
PCSK9 plays a significant role in cholesterol homeostasis and lipid transport in intestinal epithelial cells. Levy E, Ben Djoudi Ouadda A, Spahis S, Sane AT, Garofalo C, Grenier É, Emonnot L, Yara S, Couture P, Beaulieu JF, Ménard D, Seidah NG, Elchebly M. Atherosclerosis. 2013 Apr;227(2):297-306. https://pubmed.ncbi.nlm.nih.gov/23422832/
Iron-ascorbate-mediated lipid peroxidation causes epigenetic changes in the antioxidant defense in intestinal epithelial cells: impact on inflammation. Yara S, Lavoie JC, Beaulieu JF, Delvin E, Amre D, Marcil V, Seidman E, Levy E. PLoS One. 2013 May 22;8(5):e63456. https://pubmed.ncbi.nlm.nih.gov/23717425/
AMPK in the small intestine in normal and pathophysiological conditions. Harmel E, Grenier E, Bendjoudi Ouadda A, El Chebly M, Ziv E, Beaulieu JF, Sané A, Spahis S, Laville M, Levy E. Endocrinology. 2014 Mar;155(3):873-88. https://pubmed.ncbi.nlm.nih.gov/24424053/
Sar1b transgenic male mice are more susceptible to high-fat diet-induced obesity, insulin insensitivity and intestinal chylomicron overproduction. Levy E, Spahis S, Garofalo C, Marcil V, Montoudis A, Sinnet D, Sanchez R, Peretti N, Beaulieu JF, Sane A. J Nutr Biochem. 2014 May;25(5):540-8. https://pubmed.ncbi.nlm.nih.gov/24657056/
Hepatocyte nuclear factor 4 alpha polymorphisms and the metabolic syndrome in French-Canadian youth. Marcil V, Amre D, Seidman EG, Boudreau F, Gendron FP, Ménard D, Beaulieu JF, Sinnett D, Lambert M, Levy E. PLoS One. 2015 Feb 11;10(2):e0117238. https://pubmed.ncbi.nlm.nih.gov/25671620/
Altered intestinal functions and increased local inflammation in insulin-resistant obese subjects: a gene-expression profile analysis. Veilleux A, Mayeur S, Bérubé JC, Beaulieu JF, Tremblay E, Hould FS, Bossé Y, Richard D, Levy E. BMC Gastroenterol. 2015 Sep 16;15:119. https://pubmed.ncbi.nlm.nih.gov/26376914/
Apple peel polyphenols: a key player in the prevention and treatment of experimental inflammatory bowel disease. Denis MC, Roy D, Yeganeh PR, Desjardins Y, Varin T, Haddad N, Amre D, Sané AT, Garofalo C, Furtos A, Patey N, Delvin E, Tremblay E, Marette A, Beaulieu JF, Levy E. Clin Sci (Lond). 2016 Dec 1;130(23):2217-2237. https://pubmed.ncbi.nlm.nih.gov/27630205/
Plasma Lactoferrin Levels Positively Correlate with Insulin Resistance despite an Inverse Association with Total Adiposity in Lean and Severely Obese Patients. Mayeur S, Veilleux A, Pouliot Y, Lamarche B, Beaulieu JF, Hould FS, Richard D, Tchernof A, Levy E. PLoS One. 2016 Nov 30;11(11):e0166138. https://pubmed.ncbi.nlm.nih.gov/27902700/
Apple peel polyphenols reduce mitochondrial dysfunction in mice with DSS-induced ulcerative colitis. Yeganeh PR, Leahy J, Spahis S, Patey N, Desjardins Y, Roy D, Delvin E, Garofalo C, Leduc-Gaudet JP, St-Pierre D, Beaulieu JF, Marette A, Gouspillou G, Levy E. J Nutr Biochem. 2018 Jul;57:56-66. https://pubmed.ncbi.nlm.nih.gov/29674247/
From Congenital Disorders of Fat Malabsorption to Understanding Intra-Enterocyte Mechanisms Behind Chylomicron Assembly and Secretion. Levy E, Beaulieu JF, Spahis S. Front Physiol. 2021 Jan 27;12:629222. https://pubmed.ncbi.nlm.nih.gov/33584351/
Sar1b mutant mice recapitulate gastrointestinal abnormalities associated with chylomicron retention disease. Auclair N, Sané AT, Ahmarani L, Patey N, Beaulieu JF, Peretti N, Spahis S, Levy E. J Lipid Res. 2021;62:100085. https://pubmed.ncbi.nlm.nih.gov/33964306/
High-fat diet reveals the impact of Sar1b defects on lipid and lipoprotein profile and cholesterol metabolism. Auclair N, Sané AT, Ahmarani L, Ould-Chikh NE, Patey N, Beaulieu JF, Delvin E, Spahis S, Levy E. J Lipid Res. 2023 Sep;64(9):100423. https://pubmed.ncbi.nlm.nih.gov/37558128/
L’identification des marqueurs pour l’étude du développement chez la souris
Collaboration avec l’équipe de Raymond Calvert pour la génération d’anticorps monoclonaux en vue de caractériser des nouveaux marqueurs de développement.
Developmental expression of two antigens associated with mouse intestinal crypts. Beaulieu JF, Millane G, Calvert R. Dev Dyn. 1992 Apr;193(4):325-31. https://pubmed.ncbi.nlm.nih.gov/1511172/
An intestinal secretory protein is found in most glands associated with the gastrointestinal tract: von Ebner’s and salivary glands, gallbladder, and pancreas. Calvert R, Millane G, Pothier P, Beaulieu JF. J Histochem Cytochem. 1993 Aug;41(8):1223-31. https://pubmed.ncbi.nlm.nih.gov/8331286/
Immunolocalization of a mesenchymal antigen specific to the gastrointestinal tract. Calvert R, Millane G, Beaulieu JF. Anat Rec. 1994 Nov;240(3):358-66. https://pubmed.ncbi.nlm.nih.gov/7825732/
Les cellules gastriques
Collaboration avec l’équipe de Daniel Ménard pour la caractérisation de modèles de culture cellulaire gastrique.
A new primary culture system representative of the human gastric epithelium. Basque JR, Chailler P, Perreault N, Beaulieu JF, Ménard D. Exp Cell Res. 1999 Dec 15;253(2):493-502. https://pubmed.ncbi.nlm.nih.gov/10585272/
Expression of integrin subunits correlates with differentiation of epithelial cell lineages in developing human gastric mucosa. Chénard M, Basque JR, Chailler P, Tremblay E, Beaulieu JF, Ménard D. Anat Embryol (Berl). 2000 Sep;202(3):223-33. doi: 10.1007/s004290000104. https://pubmed.ncbi.nlm.nih.gov/10994995/
Epidermal growth factor receptor-dependent PI3K-activation promotes restitution of wounded human gastric epithelial monolayers. Tétreault MP, Chailler P, Beaulieu JF, Rivard N, Ménard D. J Cell Physiol. 2008 Feb;214(2):545-57. https://pubmed.ncbi.nlm.nih.gov/17708540/
Specific signaling cascades involved in cell spreading during healing of micro-wounded gastric epithelial monolayers. Tétreault MP, Chailler P, Beaulieu JF, Rivard N, Ménard D. J Cell Biochem. 2008 Dec 1;105(5):1240-9. https://pubmed.ncbi.nlm.nih.gov/18802922/
Isolation and functional studies of human fetal gastric epithelium in primary culture. Chailler P, Beaulieu JF, Ménard D. Methods Mol Biol. 2012;806:137-55. https://pubmed.ncbi.nlm.nih.gov/22057450/
L’anoïkose et l’apoptose dans les cellules intestinales
Une collaboration de longue date avec l’équipe de Pierre H. Vachon pour l’utilisation de modèles cellulaires en vue de caractériser les effets d’une perte d’interaction cellulaire avec la matrice sur des cellules normales et cancéreuses.
Differentiation state-selective roles of p38 isoforms in human intestinal epithelial cell anoikis. Vachon PH, Harnois C, Grenier A, Dufour G, Bouchard V, Han J, Landry J, Beaulieu JF, Vézina A, Dydensborg AB, Gauthier R, Côté A, Drolet JF, Lareau F. Gastroenterology. 2002 Dec;123(6):1980-91. https://pubmed.ncbi.nlm.nih.gov/12454855/
Human intestinal epithelial crypt cell survival and death: Complex modulations of Bcl-2 homologs by Fak, PI3-K/Akt-1, MEK/Erk, and p38 signaling pathways. Harnois C, Demers MJ, Bouchard V, Vallée K, Gagné D, Fujita N, Tsuruo T, Vézina A, Beaulieu JF, Côté A, Vachon PH. J Cell Physiol. 2004 Feb;198(2):209-22. https://pubmed.ncbi.nlm.nih.gov/14603523/
Proinflammatory cytokines TNF-alpha and IFN-gamma alter laminin expression under an apoptosis-independent mechanism in human intestinal epithelial cells. Francoeur C, Escaffit F, Vachon PH, Beaulieu JF. Am J Physiol Gastrointest Liver Physiol. 2004 Sep;287(3):G592-8. https://pubmed.ncbi.nlm.nih.gov/15087281/
Human intestinal epithelial cell survival and anoikis. Differentiation state-distinct regulation and roles of protein kinase B/Akt isoforms. Dufour G, Demers MJ, Gagné D, Dydensborg AB, Teller IC, Bouchard V, Degongre I, Beaulieu JF, Cheng JQ, Fujita N, Tsuruo T, Vallée K, Vachon PH. J Biol Chem. 2004 Oct 15;279(42):44113-22. https://pubmed.ncbi.nlm.nih.gov/15299029/
Fak/Src signaling in human intestinal epithelial cell survival and anoikis: differentiation state-specific uncoupling with the PI3-K/Akt-1 and MEK/Erk pathways. Bouchard V, Demers MJ, Thibodeau S, Laquerre V, Fujita N, Tsuruo T, Beaulieu JF, Gauthier R, Vézina A, Villeneuve L, Vachon PH. J Cell Physiol. 2007 Sep;212(3):717-28. https://pubmed.ncbi.nlm.nih.gov/17443665/
Integrin/Fak/Src-mediated regulation of cell survival and anoikis in human intestinal epithelial crypt cells: selective engagement and roles of PI3-K isoform complexes. Beauséjour M, Noël D, Thibodeau S, Bouchard V, Harnois C, Beaulieu JF, Demers MJ, Vachon PH. Apoptosis. 2012 Jun;17(6):566-78. https://pubmed.ncbi.nlm.nih.gov/22402981/
Suppression of anoikis in human intestinal epithelial cells: differentiation state-selective roles of α2β1, α3β1, α5β1, and α6β4 integrins. Beauséjour M, Thibodeau S, Demers MJ, Bouchard V, Gauthier R, Beaulieu JF, Vachon PH. BMC Cell Biol. 2013 Dec 1;14:53. https://pubmed.ncbi.nlm.nih.gov/24289209/
Le développement de nouveaux modèles cellulaires pour l’épithélium intestinal
Collaboration avec le Dr Andrea Quaroni et diverses initiatives du laboratoire qui ont mené à la caractérisation et à la validation de plusieurs modèles cellulaires qui se sont révélés essentiels aux études visant à mieux comprendre le fonctionnement de l’épithélium intestinal humain.
Clonal analysis of sucrase-isomaltase expression in the human colon adenocarcinoma Caco-2 cells. Beaulieu JF, Quaroni A. Biochem J. 1991 Dec 15;280 ( Pt 3)(Pt 3):599-608. https://pubmed.ncbi.nlm.nih.gov/1764023/
Transient mosaic patterns of morphological and functional differentiation in the Caco-2 cell line. Vachon PH, Beaulieu JF. Gastroenterology. 1992 Aug;103(2):414-23. https://pubmed.ncbi.nlm.nih.gov/1634060/
Basement membrane formation and re-distribution of the beta 1 integrins in a human intestinal co-culture system. Vachon PH, Durand J, Beaulieu JF. Anat Rec. 1993 Apr;235(4):567-76. https://pubmed.ncbi.nlm.nih.gov/8465988/
Uncoordinated, transient mosaic patterns of intestinal hydrolase expression in differentiating human enterocytes. Vachon PH, Perreault N, Magny P, Beaulieu JF. J Cell Physiol. 1996 Jan;166(1):198-207. https://pubmed.ncbi.nlm.nih.gov/8557768/
Use of the dissociating enzyme thermolysin to generate viable human normal intestinal epithelial cell cultures. Perreault N, Beaulieu JF. Exp Cell Res. 1996 May 1;224(2):354-64. https://pubmed.ncbi.nlm.nih.gov/8612712/
Cell dynamics and differentiation of conditionally immortalized human intestinal epithelial cells. Quaroni A, Beaulieu JF. Gastroenterology. 1997 Oct;113(4):1198-213. https://pubmed.ncbi.nlm.nih.gov/9322515/
Human cell models to study small intestinal functions: recapitulation of the crypt-villus axis. Pageot LP, Perreault N, Basora N, Francoeur C, Magny P, Beaulieu JF. Microsc Res Tech. 2000 May 15;49(4):394-406. https://pubmed.ncbi.nlm.nih.gov/10820523/
Intestinal epithelial wound healing assay in an epithelial-mesenchymal co-culture system. Seltana A, Basora N, Beaulieu JF. Wound Repair Regen. 2010 Jan-Feb;18(1):114-22. https://pubmed.ncbi.nlm.nih.gov/20082684/
Isolation, characterization, and culture of normal human intestinal crypt and villus cells. Beaulieu JF, Ménard D. Methods Mol Biol. 2012;806:157-73. https://pubmed.ncbi.nlm.nih.gov/22057451/
Modulation of stemness in a human normal intestinal epithelial crypt cell line by activation of the WNT signaling pathway. Guezguez A, Paré F, Benoit YD, Basora N, Beaulieu JF. Exp Cell Res. 2014 Apr 1;322(2):355-64. https://pubmed.ncbi.nlm.nih.gov/24534551/
Primary Cilium Identifies a Quiescent Cell Population in the Human Intestinal Crypt. Sénicourt B, Cloutier G, Basora N, Fallah S, Laniel A, Lavoie C, Beaulieu JF. Cells. 2023 Mar 31;12(7):1059. https://pubmed.ncbi.nlm.nih.gov/37048132/
L’utilisation de modèles cellulaires pour mieux comprendre la réponse cellulaire épithéliale dans l’intestin dans les cas de maladie de Crohn.
Une collaboration avec l’équipe de Frank Ruemmele de l’Hôpital Necker Enfants malades, Paris, France pour l’utilisation des cellules HIEC-6.
The susceptibility to Fas-induced apoptosis in normal enterocytes is regulated on the level of cIAP1 and 2. Ruemmele FM, Beaulieu JF, O’Connell J, Bennett MW, Seidman EG, Lentze MJ. Biochem Biophys Res Commun. 2002 Feb 1;290(4):1308-14. https://pubmed.ncbi.nlm.nih.gov/11812006/
Lipopolysaccharide modulation of normal enterocyte turnover by toll-like receptors is mediated by endogenously produced tumour necrosis factor alpha. Ruemmele FM, Beaulieu JF, Dionne S, Levy E, Seidman EG, Cerf-Bensussan N, Lentze MJ. Gut. 2002 Dec;51(6):842-8. https://pubmed.ncbi.nlm.nih.gov/12427787/
Interleukin-17 is a potent immuno-modulator and regulator of normal human intestinal epithelial cell growth. Schwartz S, Beaulieu JF, Ruemmele FM. Biochem Biophys Res Commun. 2005 Nov 18;337(2):505-9. https://pubmed.ncbi.nlm.nih.gov/16198312/
Implication of TNF-related apoptosis-inducing ligand in inflammatory intestinal epithelial lesions. Begue B, Wajant H, Bambou JC, Dubuquoy L, Siegmund D, Beaulieu JF, Canioni D, Berrebi D, Brousse N, Desreumaux P, Schmitz J, Lentze MJ, Goulet O, Cerf-Bensussan N, Ruemmele FM. Gastroenterology. 2006 Jun;130(7):1962-74. https://pubmed.ncbi.nlm.nih.gov/16762619/
Microbial induction of CARD15 expression in intestinal epithelial cells via toll-like receptor 5 triggers an antibacterial response loop. Begue B, Dumant C, Bambou JC, Beaulieu JF, Chamaillard M, Hugot JP, Goulet O, Schmitz J, Philpott DJ, Cerf-Bensussan N, Ruemmele FM. J Cell Physiol. 2006 Nov;209(2):241-52. https://pubmed.ncbi.nlm.nih.gov/16897777/
Les applications et limites du qPCR
Collaboration avec S.A. Bustin dans le cadre du comité éditorial de journal BMC Mol Biol pour améliorer le guide pour la réalisation de qPCR.
Normalizing genes for quantitative RT-PCR in differentiating human intestinal epithelial cells and adenocarcinomas of the colon. Dydensborg AB, Herring E, Auclair J, Tremblay E, Beaulieu JF. Am J Physiol Gastrointest Liver Physiol. 2006 May;290(5):G1067-74. https://pubmed.ncbi.nlm.nih.gov/16399877/
MIQE précis: Practical implementation of minimum standard guidelines for fluorescence-based quantitative real-time PCR experiments. Bustin SA, Beaulieu JF, Huggett J, Jaggi R, Kibenge FS, Olsvik PA, Penning LC, Toegel S. BMC Mol Biol. 2010 Sep 21;11:74. https://pubmed.ncbi.nlm.nih.gov/20858237/
The need for transparency and good practices in the qPCR literature. Bustin SA et al., Nat Methods. 2013 Nov;10(11):1063-7. https://pubmed.ncbi.nlm.nih.gov/24173381/
La maladie cœliaque
Une collaboration avec le laboratoire de Donatella Barisani (Université de Milano-Biocca, Italie) pour l’étude des interactions entre les peptides dérivés du gluten et les nanoparticules alimentaires.
Gliadin, through the Activation of Innate Immunity, Triggers lncRNA NEAT1 Expression in Celiac Disease Duodenal Mucosa. Gnodi E, Mancuso C, Elli L, Ballarini E, Meneveri R, Beaulieu JF, Barisani D. Int J Mol Sci. 2021 Jan 28;22(3):1289. https://pubmed.ncbi.nlm.nih.gov/33525473/
Dietary Nanoparticles Interact with Gluten Peptides and Alter the Intestinal Homeostasis Increasing the Risk of Celiac Disease. Mancuso C, Re F, Rivolta I, Elli L, Gnodi E, Beaulieu JF, Barisani D. Int J Mol Sci. 2021 Jun 5;22(11):6102. https://pubmed.ncbi.nlm.nih.gov/34198897/
The Combination of Gold and Silver Food Nanoparticles with Gluten Peptides Alters the Autophagic Pathway in Intestinal Crypt-like Cells. Mancuso C, Tremblay E, Gnodi E, Jean S, Beaulieu JF, Barisani D. Int J Mol Sci. 2023 Aug 22;24(17):13040. https://pubmed.ncbi.nlm.nih.gov/37685847/
Les microvésicules dans le lait, les microRNAs et leurs effets sur la muqueuse intestinale
Un projet mené en collaboration avec Emanuel Ferretti, Illimar Altosaar et Marceline Côté (Université d’Ottawa). À suivre.
Review of Methodological Approaches to Human Milk Small Extracellular Vesicle Proteomics. Kraft J, Ferretti E, Chung M, Beaulieu JF, Altosaar I. Biomolecules. 2021 Jun 3;11(6):833. https://pubmed.ncbi.nlm.nih.gov/34204944/
Gestational age at birth influences protein and RNA content in human milk extracellular vesicles. Vahkal B, Altosaar I, Tremblay E, Gagné D, Huttman N, Minic Z, Coté M, Blais A, Beaulieu JF, Ferretti E., J Extracellular Bio. 2024: 3: e128. https://doi.org/10.1002/jex2.128
Laboratoire de physiopathologie intestinale/ Laboratory of Intestinal Physiopathology 